Stat5 reporter assay

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August undefined, 2024

Web6.2 STAT5 Signaling. STAT5 was shown to be a mediator of erythropoietin-induced neuroprotection in a rat model of global cerebral ischemia (Zhang, Wang, Cao, Gao, & … WebIn an attempt to use the cells for large-scale chemical screens to identify JAK3 inhibitors, we established a cell line, 32D/IL-2Rβ/6xSTAT5, stably expressing a STAT5 reporter gene. Treatment of this cell line with IL-2 or IL-3 dramatically increased the reporter activity in a high-throughput format.

STAT5 Reporter (Luc) – Ba/F3 Cell line - BPS Bioscience

WebFeb 2, 2024 · Two duplicate plates were set up, one for SEAP reporter assay and one for CellTiter-Glo cell viability assay. SEAP was determined using QUANTI-Blue (InvivoGen, rep-qbs). WebThis assay utilized the growth factor-dependent human erythroleukemia TF1 cell line engineered to express a green fluorescent protein-STAT5 fusion protein. Furthermore, a complementary beta-lactamase reporter gene assay was developed to analyze the transcriptional activity of STAT5 downstream of JAK2 in TF1 cells. new job astrology

A cellular reporter assay to monitor insulin receptor kinase activity ...

WebMay 12, 2010 · A skewed ratio of pro-inflammatory to anti-inflammatory cytokines, elevated growth factor synthesis and T- and B-lymphocyte activation are 3 hallmarks of rheumatoid arthritis (RA) pathology. Interleukin-6 (IL-6), IL-7, IL-17, IL-12/IL-23 and growth factors, granulocyte macrophage-colony stimulating factor, IL-3, and erythropoietin activate the … WebMay 1, 2011 · One day after transfection, β-galactosidase reporter assays were performed. Specific activities from cells transfected with p ... Stat5 reporter gene expression in tissue sections from liver, kidney, spleen, thymus, and uterus. Indicated organs were isolated from C57BL/6 wild-type mice (row 1) and Stat5-REIN reporter mice (rows 2 and 3 ... WebThe STAT5 Reporter (Luc)-Ba/F3 cell line is designed for monitoring STAT5 signal transduction pathways. It contains a firefly luciferase gene driven by the STAT5 response … The One-Step™ Luciferase Assay System is designed for high-throughput, sensitive … Recombinant Sortase A, His-tag (BPS Bioscience #71086) dose-dependent … in this matter or on this matter grammar

Development of a STAT5 phosphorylation assay as a rapid

Frontiers Evaluating STAT5 Phosphorylation as a Mean …

WebMar 19, 2013 · Reporter assay to monitor oncogenic FLT3-ITD activity. As FLT3-ITD strongly activates STAT5, 3, 4, 12, 13, 20, 24, 26 we employed a FLT3-ITD-driven STAT5 activation reporter assay for the screen ... WebOct 1, 2004 · IL-4/GFP reporter mice ... This suggests that STAT5 may be involved in asthma through mast cell pathogenesis (8,26) and there have been several ... Enzyme‐linked … new job at 3 weeks still no trainingWebHygromycin resistance selectable marker means choosing transient transfection or generating a stable cell line Choose a response element ISRE SBE SIE STAT5 RE Size 20µg Catalog number selected: E4041 $ 435.00 Your price: Log in Add to Cart Add to Helix Cytokine Signaling Pathway Analysis Response Element Firefly Luciferase Vectors … in this meaning

"WebThe following assays are designed for 96-well format. To perform the assay in a different format, the cell number and reagent volume should be scaled appropriately. A. Dose response of STAT5 reporter (Luc) -U937 cells to human GM-CSF 1. Harvest STAT5 reporter (Luc)-U937 cells from culture in the Growth Medium 8A and seed cells at a " - Stat5 reporter assay

Stat5 reporter assay

Frontiers Evaluating STAT5 Phosphorylation as a Mean …

WebMay 25, 2000 · It is clear, however, that Stat5 tetramer formation is not critical for Stat5-mediated transcription of reporter constructs containing high affinity GAS motifs (John et al., 1999), a finding that ... WebAssay Interferences Interference testing should be relevant to the patient population that will be tested with a given assay endogenous substances - highest reported clinically relevant …

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WebLike the Kinase receptor activation (KIRA) assay, developed by Sadick et al. [1], the STAT5 phosphorylation assay was performed using two separate microtiter plates: the first one … WebReporter Vector primer 4 (RVprimer4) binding region 4171–4190 Sequence information for the pGL4 Vectors is available online at: www.promega.com/vectors/ Example Protocol In …

WebMoreover, bioinformatics analyses and luciferase reporter assay indicated that exosomal miR-182-5p was bound to PPARG 3' UTR sequence and inhibited the expression of PPARG. Collectively, our findings provided a new role of EPCs-EXO in the clinical treatment of diabetic skin wounds. ... (STAT5) to control EPC cell-cycle progression 44. In ... WebReadily assessable STAT5-inducible SEAP reporter activity The stability for 20 passages has been verified Functionally tested and guaranteed mycoplasma-free Applications of HEK-Blue™ IL-9 cells: Detection of human IL-9 (100 pg/ml - 100 ng/ml) and murine IL-9 (30 pg/ml -100 ng/ml) Screening of anti-IL-9 and anti-IL-9R antibodies References: 1.

WebTo analyze STAT5A phosphorylation: based on the following gating strategy (1) forward scatter (FSC) vs. side scatter (SSC) and (2) CD3 vs. SSC, the T cells (CD3 +) were separated ( Supplementary Figure 1 ). Now, after clear … WebAug 15, 2003 · Stat5 deficiency reduces survival and proliferation of developing mast cells. (A) Wild-type and Stat5-deficient bone marrow cells were cultured for the indicated times …

WebSTAT5 reporter system is designed to monitor the STAT5 transcriptional activity. STAT5 reporter assay services include screening of STAT5 agonists/antagonists, EC50/IC50 evaluation of those molecules, and functional analysis of STAT5 signaling pathways. Assay results will contain a written report and downloadable Excel- or Prism-based data.

Webperforming dual-luciferase reporter assays. 3) Firefly Luciferase (Fluc) Lentivirus (BPS Bioscience, #79692-G, #79692-H, #79692-P): ... STAT5 Reporter (Luc)-BaF3 cell line 79772 2 vials ONE-Step™ Luciferase Assay System 60690-1 10 ml ONE-Step™ Luciferase Assay System 60690-2 100 ml ... new job at 35WebMay 25, 2000 · The finding that glucocorticoid receptor (GR) is associated with Stat5 came from studies on the β-casein gene. Upon interaction with its steroid ligand, GR binds to specific DNA sequence, the... in this matter sentenceWebAssay Entry Cell-based terbium phospho-specific activity (stable) Cell State Dividing Cells Detection Method Fluorescent Gene Symbol STAT5A Optimal Response GM-CSF (EC50 = … new job at 58WebMar 10, 2011 · The STAT5 reporter assays in a 96-well plate format The 32D/IL-2Rβ/6xSTAT5 reporter cells were deprived of WEHI-3 cell-conditioned medium for 6 h. … new job at 50WebWe established and validated a rapid, sensitive, flow cytometric based pSTAT5A assay to detect T cell proliferation. We showed that there was a strong correlation between the early CD3/CD28 or polyclonal mitogen … new job at 59WebA highly sensitive method for determination of insulin receptor (IR) kinase activity in whole cells, which is based on a STAT5 (signal transducer and activator of transcription 5)-dependent reporter gene assay, has been developed. new job at 40WebFeb 24, 2024 · General strategy for the design of the reporter cell line: [a] signal through most of the immunologically relevant receptors results in the activation of NF-κB 23, 30, … in this meaningful moment