Phosphotyrosine antibody
WebAnti-Phosphotyrosine Antibody, clone 4G10 detects tyrosine phosphorylated proteins in all species. This unique monoclonal antibody is validated for use in IC, IH, IP, WB and is … WebJan 12, 2012 · (a) Detection of biotinylated 4G10 (anti-Phosphotyrosine) antibody using streptavidin conjugated 655 nm QDs in a triton permeabilized embryo. Specific staining can be seen at the cell-cell boundaries of the ectodermal cells of the fin (upper part of the image) but not in the deep tissues (somites at the bottom part of the image).
Phosphotyrosine antibody
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WebR&D Systems now offers Phospho-Tyrosine antibodies suitable for all applications typically used to analyze protein tyrosine phosphorylation, including Western blotting, immunoprecipitation, immunohistochemistry, and ELISA. Figure 1. Lysates from human A431 cells were either untreated (-) or treated with 50 µM pervanadate (PV) for 15 … WebFeb 13, 2024 · Phosphotyrosine Antibodies Antibodies that detect Phosphotyrosine can be used in several scientific applications, including Western Blot, ELISA, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry. These antibodies target …
WebApplication. Anti-Phosphoserine Antibody detects level of Phosphoserine & has been published & validated for use in ELISA, IH, IP & WB. 10-20 μg anti-pS/mg protein was used from a previous lot. Note that this will immunoprecipitate all phosphoserine proteins in the extracts. If using purified protein exacts containing only the protein of ... Web4G10 Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) detects levels of Phosphotyrosine proteins & has been published & validated for use in WB, ICC, ELISA, IHC, IF, Flow & More>> MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. SDS CoA References
WebSee all Phosphotyrosine primary antibodies Description Rabbit monoclonal [EPR16871] to Phosphotyrosine Host species Rabbit Tested applications Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP, ELISA, Dot blot more details Species reactivity Reacts with: Species independent Immunogen Synthetic peptide. WebPhosphotyrosine Antibody (03-7700) in ELISA Direct ELISA analysis of Phosphotyrosine was performed by coating wells of a 96-well plate with 100 µL per well of Phosphotyrosine or Phosphoserine diluted in …
WebThe fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations …
WebApr 25, 1991 · The detection of this group of phosphoproteins by the antibody was prevented by coincubation with an excess of phosphotyrosine but not with an excess of phosphoserine or phosphothreonine. The BK-promoted increase in phosphorylation was rapid and transient with the peak response apparent following BK exposure for 1 min. highest cia rankWebPhosphotyrosine antibody specifically binds to phosphotyrosine residues. It is a valuablel tool to analyze tyrosine phosphorylation and monitor the activity of tyrosine kinase in high throughput drug discovery. THE™ Phosphotyrosine Antibody (E10) plus recognizes a broadspectrum of phosphotyrosine-containing proteins from numerous species ... how full is the colorado river nowWebImmunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Phosphotyrosine antibody [PY20] (ab197508) ab197508 staining Phosphotyrosine in PC12 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. highest cholesterol reading ever recordedWebDownload Zoom Anti-Phosphotyrosine Antibody, clone PY20 is an antibody against Phosphotyrosine for use in ELISA, IP & WB. Anti-Phosphotyrosine Antibody, clone PY20 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. SDS CoA References how full is the colorado riverWebThis product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility. - Improved sensitivity and … highest chords victory worshipWebAnti-Phosphotyrosine Antibody, clone PY20 is an antibody against Phosphotyrosine for use in ELISA, IP & WB. Anti-Phosphotyrosine Antibody, clone PY20 MSDS (material safety … highest cholesterol levels in the worldWebAntibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function. Specificity The immunoreactivity of the antibody is totally inhibited by the use of 100 mM phenyl phosphate, a phosphotyrosine analog. how full is your bucket activity